Breakthrough in plant breeding

Grafting and mobile CRISPR for genome editing in plants

The blueprint of the CRISPR/Cas9 gene scissors is transported as RNA from the ro
The blueprint of the CRISPR/Cas9 gene scissors is transported as RNA from the rootstock of a gene-modified plant to the grafted shoot of an unmodified plant. There, the gene scissor protein is built with the help of the RNA. The gene scissor protein edits specific genes in flowers. Plants in the next generation carry the desired gene modification. RTDS Association

A ground-breaking twist to the CRISPR tool - aka "genetic scissors" - is being put to use to edit plant genomes by scientists from the Max Planck Institute of Molecular Plant Physiology, signalling a methodology change. The discovery genetically stable commercial crop varieties by combining grafting with a ’mobile’ CRISPR tool.

An unmodified shoot is grafted onto roots that contain a mobile CRISPR/Cas9, which allows the genetic scissor to move from the root into the shoot. There it edits the plant DNA but leaves no trace of itself in the next generation of plants. ...

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